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- W2372019937 abstract "Objective: To construct the recombinant expression vector of Cuedc2 and detect its expression in HEK293 cells. Methods: Total RNA was isolated from ovarian cancer cells and RT-PCR was conducted to acquire the cDNA. A 952 bp fragment containing the coding region of Cuedc2 was amplified by PCR and the resulting product was purified. Then this fragment was used as new template and the coding region of Cuedc2(864 bp)was amplified, purified and inserted into the BamHI and Hind Ⅲ sites of the pcDNA3.1myc-his(-)A expression vector. The sequence was confirmed by colony PCR and DNA sequencing. In the end, pcDNA3.1 myc-his(-)A-Cuedc2 was transiently transfected into HEK293 cells and the expression of this new construct was detected by western blot. Results: The full length coding region of Cuedc2 was obtained and confirmed, the expression of Cuedc2 was detected successfully in HEK293 cells. Conclusion: The eukaryotic expression vector of Cuedc2 had been successfully constructed, which would provide a useful tool for designing an in-depth investigation of the role of Cuedc2 in tumorigenesis." @default.
- W2372019937 created "2016-06-24" @default.
- W2372019937 creator A5091083830 @default.
- W2372019937 date "2014-01-01" @default.
- W2372019937 modified "2023-09-23" @default.
- W2372019937 title "Construction of Human Cuedc2 eukaryotic Expression Vector and Expression of Cuedc2 in HEK293 Cells" @default.
- W2372019937 hasPublicationYear "2014" @default.
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