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- W2372032441 abstract "Objective To obtain the monoclonal antibodies against the protein of ginkgo diterpene lactones meglumine injection.Methods After total proteins of ginkgo leaf running two-dimensional electrophoresis,the protein spots were digged and analyzed by mass spectrometry(MS).According to the result of MS,the polypeptide was synthesized and used to immunize the BALB/c mice.The indirect ELISA was utilized to develop monoclonal antibody after cell fusion between SP2/0 cells and spleen cells from the immunized BALB/c mice.Results The immunoglobulin subtypes of five monoclonal antibodies were IgM and light chain was Kappa identified with a commercial capture-ELISA kit.Western blotting analysis also indicated that the monoclonal antibodies were specific to the protein of ginkgo leaf.Conclusion The experiment would be helpful for the establishment of detection method specific to the proteins of ginkgo diterpene lactones meglumine injection in the future study." @default.
- W2372032441 created "2016-06-24" @default.
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- W2372032441 date "2013-01-01" @default.
- W2372032441 modified "2023-09-23" @default.
- W2372032441 title "Preparation of monoclonal antibody against proteins of ginkgo diterpene lactones meglumine injection" @default.
- W2372032441 hasPublicationYear "2013" @default.
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