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- W2372037900 abstract "E.coli JM101,DH5 α and C600 were transfor med with plasmids pLT and pLTR72 respectively and then cultured at 37℃ for abou t 24 hours in growth media CAYE-2 and LB,respectively.The whole cell lysates of all these cultured E.coli were prepared by frozen-thaw and sonication.The expressed products in the lysates were tested by GM1-ELISA.The results showed t hat the mutants were expressed well when they were transformed into the three E. coli strains and cultured in either CAYE-2 or LB media,but the best choice for the expression of these mutants was in the host of DH5 α and cultured in CAYE - 2 medium;the results of GM1-ELISA showed that the mutants had GM1 binding activ ity.The mutants and LT were expressed from the host of DH5 α harbouring the pl asmids pLTR72 and pLT were isolated and purified from their whole-cell lysate r espectively,by the method of (NH 4) 2SO 4 precipitation,Gel filtration chroma tography,maltose elution,all the pooled samples were tested by SDS-PAGE and den sitometric scan analysis.The results showed that the contents of LTR72 and LT i n the samples were 96% and 94% of the total amount of the sample protein respecti vely.LTR72 and LT consisted of two subunits,and they had the same construction,t hat was 1A∶5B." @default.
- W2372037900 created "2016-06-24" @default.
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- W2372037900 date "2003-01-01" @default.
- W2372037900 modified "2023-09-24" @default.
- W2372037900 title "Expression and Evaluation of the Mutants of Escherichia coli Heat- labile Enterotoxin" @default.
- W2372037900 hasPublicationYear "2003" @default.
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