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- W2372049084 abstract "Objective To detect the pathogen EV71 of HFMD by molecular biological techniques and offer the foundation to clinical diagnosis.Methods According to the Ministry of Health issued by the Hand,Foot and Mouth Disease Control and Prevention Guide(2008),265 dejecta of HFMD in May 2008 in Shenzhen Donghu Hospital were collected.RT-PCR was applied to detect the pathogen EV71 of HFMD and the products were sequenced and analyzed in GenBank to verify the accuracy.Results Specific DNA fragments of EV71 VP1 district were detected by RT-PCR,24 percent of the dejecta were positive.DNA fragments sequencing verified it was EV71 VP1 gene and the accuracy of RT-PCR was up to 70 percent(14/21).Conclusions RT-PCR can be used to detect pathogen EV71 of HFMD in clinical diagnosis." @default.
- W2372049084 created "2016-06-24" @default.
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- W2372049084 date "2008-01-01" @default.
- W2372049084 modified "2023-09-24" @default.
- W2372049084 title "Detection of EV71 as the pathogen of HFMD by RT-PCR" @default.
- W2372049084 hasPublicationYear "2008" @default.
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