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- W2372075915 abstract "Eleven populations of cereal cyst nematode(CCN) from wheat were collected in collected in Qinghai and Shaanxi Provinc,all morphologically identified as Heterodera avenae.The rDNA-ITS regions of the populations were amplified with the universal primers AB28 and TW81,a fragment of approximately 1 060 bp was yielded.The fragments were digested with Hinf Ⅰ,TaqⅠ,Hpa Ⅱ,Hae Ⅲ,Pst Ⅰ and Alu Ⅰ.The identical digestion patterns were obtained from CCN populations(YBT10A,HY65A,HY61B,ZHZ162B,HY5B,HHX8A,GH132A,HY92A,HY127B and DT142A) of Qinghai and which were the same species.The digestion patterns were the same between YL4A population of Shaanxi and the ones of Qinghai by Hae Ⅲ,Hinf Ⅰ and Hpa Ⅱ.The digestion patterns of Pst Ⅰ and Alu Ⅰ showed that the YL4A CCN population was differed from the ones of Qinghai,because it had an extra 1 060 bp fragment.The Taq Ⅰ restriction pattern of YL4A CCN population was complex.The obvious difference could be observed in the Taq Ⅰ RFLP pattern,a 520 bp fragment was not presented in the Qinghai CCN populations but in the Henan populations.According to the known conclusions,the analysis results restriction profiles showed that 10 populations of Qinghai were the identical RFLP patterns of H.avenae from China.Alu Ⅰ profile of YL4A population was the same as H.avenae from France,then digestion of YL4A population by Pst Ⅰ and Taq Ⅰ were different with either members of Avenae group because of its complex profiles.The Henan CCN populations were the similar as Qinghai ones except RFLP profile by Taq Ⅰ,and identical with H.avenae from Australia." @default.
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- W2372075915 date "2011-01-01" @default.
- W2372075915 modified "2023-09-28" @default.
- W2372075915 title "rDNA-ITS restriction fragment length polymorphism of cereal cyst nematodes in some regions of Qinghai,Shaanxi and Henan Province,China" @default.
- W2372075915 hasPublicationYear "2011" @default.
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