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- W2372094797 abstract "【Objective】To investigate proliferation and telomere length in rat fibroblasts of different ages,and to provide the evidence for the selection of nulear donor cells used in therapeutic cloning.【Methods】Cell growth curve was described by MTT assay and cell cycle was detected through flow cytometry.PCR-ELISA assay was used to test telomerase activity and telomere terminal restriction fragment(TRF) length was measured by Southern blotting in the research.【Results】①The proliferation ability of rat fibroblasts was decreased with age.②The population doubling time of passage 10 was longer than that of passage 1 in fibroblasts of 1-year group and over 2-year group.③Telomere TRF length of fibroblasts was shortened with age and cultured time,especially the latter.④Telomerase activity was not found in fibroblasts of each group.【Conclusion】①The proliferation ability of the fibroblasts was decreased with the age and the culture time.②The telomere TRF length of the fibroblasts was shortened with age and cultured time." @default.
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- W2372094797 date "2008-01-01" @default.
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- W2372094797 title "Study on Proliferation and Telomere Length of Fibroblasts in Different Age Rats" @default.
- W2372094797 hasPublicationYear "2008" @default.
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