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- W2372162225 abstract "[Objective]To explore a new method for the isolation, cultivaton, purification and identification of MSCs and observe the biological features of canine MSCs in vitro.[Methods]10 ml bone marrow was extracted from the iliac of canine. The marrow liquid were isolated with 1.073 g/ml Percoll. MSCs were obtained by removing the non-adherent cells. Then the MSCs were purified and expanded through passage in time. The growth curve was drawn and the morphology was observed under phase contrast microscope. Cell cycle and the antigen expression of MSCs were measured with FACS. [Results]The MSCs growth behavior was quite stable in IMEM containing 100 ml/L newborn bovine serum, and the growth curves of passage 1,3 and 5 were quite similar, exhibiting a large expansive potential. The FACS results showed that MSCs expressed antigen CD44,the symbol of stem cell, but did not express CD34, CD14 and HLA-DR. The cell cycle analysis showed that 82.4% of the passage 2 MSCs were in G0/G1 plate.[Conclusions]The marrow MSCs could be effectively isolated, purified and expanded by density gradient centrifugation and adhering to the culture plate." @default.
- W2372162225 created "2016-06-24" @default.
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- W2372162225 date "2009-01-01" @default.
- W2372162225 modified "2023-09-28" @default.
- W2372162225 title "Isolation,cultivation,purification and identification of canine bone marrow mesenchymal stem cells in vitro" @default.
- W2372162225 hasPublicationYear "2009" @default.
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