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- W2372180597 abstract "The auto-inducing expression of mutant human glucagon-like peptide-1 fusion protein in Escherichia coli BL21 (DE3) was studied. In order to increase the protein yield, we optimized both the auto-induction fermentation conditions and medium. The results showed that the optimal auto-induction medium is: tryptone 19.17 g/L, yeast extract 9.59 g/L, Na2HPO4 5.72 g/L, KH2PO4 5.48 g/L, (NH4)2SO4 2.66 g/L, NaCl 3.33 g/L, glycerol 2% (V/V), glucose 0.68 g/L, lactose 6.33 g/L, MgSO4 0.24 g/L. The genetically engineered strain was cultivated at 33°C, pH 7, in a 100 mL flask containing 20 mL such medium, with the inoculum size of 1%, for 12 h. Then the yield of mutant human glucagon-like pep- tide-1 fusion protein reached 348.6 mg/L." @default.
- W2372180597 created "2016-06-24" @default.
- W2372180597 creator A5020723004 @default.
- W2372180597 date "2010-01-01" @default.
- W2372180597 modified "2023-09-28" @default.
- W2372180597 title "Auto-inducing Expression Optimization of Mutant Human Glucagon-like Peptide-1 Fusion Protein in Escherichia coli BL21 (DE3)" @default.
- W2372180597 hasPublicationYear "2010" @default.
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