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- W2372187430 abstract "AIM:To construct Cdh1 small interfering RNA(siRNA)eukaryotic expression vector and evaluate its expression in Hela cells.METHODS:The interfering sequence and control sequence for Cdh1 small hairpin RNA(shRNA)were designed based on pENTRTM/H1/TO vector and the oligonucleotides were synthesized.These oligonucleotides were annealed and ligated into linearized pENTRTM/H1/TO vector respectively.After confirmation by DNA sequencing,positive recombinant plasmids were transfected into Hela cells respectively by the liposome method.Forty-eight hours later,total cellular RNA and total protein were extracted,then the expression of Cdh1 was analyzed by Real Time-PCR and Western Blot.RESULTS:DNA sequencing confirmed the successful construction of Cdh1 siRNA eukaryotic expression vector and control vector,named as pENTR/shCdh1 and pENTR/shcontrol respectively.Compared with the Hela cells transfected with pENTR/shcontrol or untransfected,the transfected pENTR/shCdh1 cells showed significant suppression in the expression of Cdh1(P0.05).CONCLUSION:The Cdh1 siRNA eukaryotic expression vector is successfully constructed and it can knockdown the expression of Cdh1 in Hela cells." @default.
- W2372187430 created "2016-06-24" @default.
- W2372187430 creator A5045732151 @default.
- W2372187430 date "2007-01-01" @default.
- W2372187430 modified "2023-10-04" @default.
- W2372187430 title "Construction and identification of Cdh1 small interfering RNA eukaryotic vector" @default.
- W2372187430 hasPublicationYear "2007" @default.
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