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- W2372203670 abstract "Objective To construct eukaryotic expression vector for human differentiation inhibitor3(Id3) fused with enhanced green fluorescent protein (EGFP) and express the fusion protein Id3-EGFP in A549 cells. Methods The human Id3 cDNA was amplified and inserted into the pEGFP-N1 vector to construct the recombinant eukaryotic expression vector pEGFP/Id3. The fusion protein Id3-EGFP was expressed in A549 cells by transfection of Id3/pEGFP into the cells. Results The pEGFP/Id3 recombinant expression vector was constructed and confirmed by DNA sequencing、enzymatic digestion and PCR identification. Both pEGFP-N1 and pEGFP/Id3 were transfected into human A549 cells by Lipofectamine~ TM 2000. Flow cytometry analysis revealed that 48 h after transfection, the proportions of EGFP+ cells of pEGFP-N1 and pEGFP/Id3 transfected cells were about 65.40% and 60.50% respectively.The cell proportions in S phase and G2 phase increased in A549 transfected with Id3/pEGFP in comparison with A549 cells transfected with pEGFG-N1 or no transfected. These findings suggested that transfection of Id3 gene into A549 induced cell proliferation by increasing the number of cells from G1 phase to G2 phases. Conclusion The construction of pEGFP/Id3 eukaryotic expression vector and expression of Id3-EGFP fusion protein in A549 cells provide a foundation for the further study of the relationship between Id3 and growth and proliferation of tumor cells." @default.
- W2372203670 created "2016-06-24" @default.
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- W2372203670 date "2006-01-01" @default.
- W2372203670 modified "2023-09-27" @default.
- W2372203670 title "Construction of Id3-EGFP eukaryotic expression vector and its expression in A549 cells" @default.
- W2372203670 hasPublicationYear "2006" @default.
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