FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2372207620> ?p ?o ?g. }

• W2372207620 endingPage "3113" @default.
• W2372207620 startingPage "3109" @default.
• W2372207620 abstract "OBJECTIVE To explore the strategy to raise both mucosal and systemic anti-HIV-1 immunity. METHODS Eighteen BALB/c rats were randomly divided into 2 groups, experimental group and control group. The experimental group were further subdivided into 4 subgroups of 3 mice: 3-dose HIV DNA vaccine group, 3-dose DNA vaccine + cholera toxin (CT) adjuvant subgroup, 1-dose recombinant Tiantan strain vaccinia-based vaccine subgroup, and 3-dose DNA vaccine + CT adjuvant + Tiantan strain vaccinia-based vaccine subgroup. The control group was subdivided into 2 subgroups of 3 mice: 3-dose DNA blank vector subgroup, and 3-dose DNA blank vector + Tiantan strain vaccinia-based vaccine subgroup. Intranasal administration of DNA vaccine-based vaccine (10 microg) was done on the days 0, 14, and 28 as the mucosal priming, and recombinant Tiantan vaccinia (1 x 10(7) PFU) was injected intramuscularly as systemic boosting on the day 42. On the day 56 the mice were killed and specimens of serum, nasopharynx wash, lung wash, and spleen were collected and splenocytes were isolated. Splenocytes were added into the phosphate-buffered saline with anti-mouse interferon-gamma (IFN-gamma) envelop antibody to count the number of spot-forming cells (SFCs). Indirect ELISA was used to detect the HIV-1 specific antibody in the nasopharynx wash and lung wash. Immunohistochemistry was used to detect the intracellular staining of IFN-gamma in the splenocytes. RESULTS The number of spot forming cells in the HIV-1 DNA vaccine + CT adjuvant group was (14 +/- 11) SFCs/10(6) splenocytes, significantly more than that of the HIV-1 DNA vaccine group [(2 +/- 1) SFCs/10(6) spleen cells (P < 0.01). The number of SFCs of the 1-dose DNA-vaccine subgroup was [(30 +/- 18) SFCs/10(6) spleen cells], significantly higher than that of the only DNS vaccine group (P < 0.01). The number of SFCs of DNA vaccine + CT adjuvant + recombinant Tiantan vaccinia-based vaccine was (61 +/- 35) SFCs/10(6) splenocytes, significantly higher than those of the other groups (all P < 0.01). Flow cytometry showed that the rate of HIV-1 Gag specific CD8(+) T cell was 1.8% +/- 1.4%. The value of specific IgG of the DNA vaccine + adjuvant + Tiantan vaccinia-based vaccine was 1.50 +/- 0.30, significantly higher than those of the blank vector, single-dose Tiantan vaccinia-based vaccine, and single-dose DNA vaccine + CT adjuvant subgroups (0.42 +/- 0.02, 0.74 +/- 0.13, and 0.75 +/- 0.02 respectively, all P < 0.05). In different subgroups the levels of specific IgA in the lung wash were all higher than those in the nasopharynx wash. The levels of specific IgA in the lung and nasopharynx wash of the DNA vaccine + CT adjuvant subgroup were higher than those of the other subgroups whether or not with boosting of Tiantan. The specific IgA levels of the groups enhanced by Tiantan vaccinia-based vaccine were all significantly higher than those of the corresponding subgroups without enhancement (all P < 0.01). The IgA level of lung wash of the DNA vaccine + CT adjuvant subgroup was 1.82 +/- 0.76, significantly higher than that of the one-dose Tiantan vaccinia-based vaccine group (0.52 +/- 0.19, P < 0.05). CONCLUSION The vaccination modality of mucosal priming and systemic boosting induces both mucosal and systemic immune responses." @default.
• W2372207620 created "2016-06-24" @default.
• W2372207620 creator A5002106678 @default.
• W2372207620 creator A5016738322 @default.
• W2372207620 creator A5025052028 @default.
• W2372207620 creator A5032393123 @default.
• W2372207620 creator A5036496332 @default.
• W2372207620 creator A5062960400 @default.
• W2372207620 creator A5071931643 @default.
• W2372207620 creator A5080499120 @default.
• W2372207620 creator A5087830342 @default.
• W2372207620 date "2006-11-28" @default.
• W2372207620 modified "2023-09-24" @default.
• W2372207620 title "[Intranasal priming with HIV DNA vaccine and systemic boosting with recombinant vaccinia induce vigorous immune responses: experiment with mice]." @default.
• W2372207620 doi "https://doi.org/10.3760/j:issn:0376-2491.2006.44.004" @default.
• W2372207620 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17313761" @default.
• W2372207620 hasPublicationYear "2006" @default.
• W2372207620 type Work @default.
• W2372207620 sameAs 2372207620 @default.
• W2372207620 citedByCount "2" @default.
• W2372207620 countsByYear W23722076202013 @default.
• W2372207620 countsByYear W23722076202022 @default.
• W2372207620 crossrefType "journal-article" @default.
• W2372207620 hasAuthorship W2372207620A5002106678 @default.
• W2372207620 hasAuthorship W2372207620A5016738322 @default.
• W2372207620 hasAuthorship W2372207620A5025052028 @default.
• W2372207620 hasAuthorship W2372207620A5032393123 @default.
• W2372207620 hasAuthorship W2372207620A5036496332 @default.
• W2372207620 hasAuthorship W2372207620A5062960400 @default.
• W2372207620 hasAuthorship W2372207620A5071931643 @default.
• W2372207620 hasAuthorship W2372207620A5080499120 @default.
• W2372207620 hasAuthorship W2372207620A5087830342 @default.
• W2372207620 hasConcept C104317684 @default.
• W2372207620 hasConcept C159047783 @default.
• W2372207620 hasConcept C167508917 @default.
• W2372207620 hasConcept C189976730 @default.
• W2372207620 hasConcept C203014093 @default.
• W2372207620 hasConcept C22070199 @default.
• W2372207620 hasConcept C2777760223 @default.
• W2372207620 hasConcept C2777863537 @default.
• W2372207620 hasConcept C2779341262 @default.
• W2372207620 hasConcept C2780801004 @default.
• W2372207620 hasConcept C2781356689 @default.
• W2372207620 hasConcept C40767141 @default.
• W2372207620 hasConcept C55493867 @default.
• W2372207620 hasConcept C71924100 @default.
• W2372207620 hasConcept C86803240 @default.
• W2372207620 hasConcept C8891405 @default.
• W2372207620 hasConceptScore W2372207620C104317684 @default.
• W2372207620 hasConceptScore W2372207620C159047783 @default.
• W2372207620 hasConceptScore W2372207620C167508917 @default.
• W2372207620 hasConceptScore W2372207620C189976730 @default.
• W2372207620 hasConceptScore W2372207620C203014093 @default.
• W2372207620 hasConceptScore W2372207620C22070199 @default.
• W2372207620 hasConceptScore W2372207620C2777760223 @default.
• W2372207620 hasConceptScore W2372207620C2777863537 @default.
• W2372207620 hasConceptScore W2372207620C2779341262 @default.
• W2372207620 hasConceptScore W2372207620C2780801004 @default.
• W2372207620 hasConceptScore W2372207620C2781356689 @default.
• W2372207620 hasConceptScore W2372207620C40767141 @default.
• W2372207620 hasConceptScore W2372207620C55493867 @default.
• W2372207620 hasConceptScore W2372207620C71924100 @default.
• W2372207620 hasConceptScore W2372207620C86803240 @default.
• W2372207620 hasConceptScore W2372207620C8891405 @default.
• W2372207620 hasIssue "44" @default.
• W2372207620 hasLocation W23722076201 @default.
• W2372207620 hasLocation W23722076202 @default.
• W2372207620 hasOpenAccess W2372207620 @default.
• W2372207620 hasPrimaryLocation W23722076201 @default.
• W2372207620 hasRelatedWork W2348020497 @default.
• W2372207620 hasRelatedWork W2354109325 @default.
• W2372207620 hasRelatedWork W2358264727 @default.
• W2372207620 hasRelatedWork W2360663767 @default.
• W2372207620 hasRelatedWork W2360733526 @default.
• W2372207620 hasRelatedWork W2365985206 @default.
• W2372207620 hasRelatedWork W2369977274 @default.
• W2372207620 hasRelatedWork W2375388602 @default.
• W2372207620 hasRelatedWork W2376615376 @default.
• W2372207620 hasRelatedWork W2381220883 @default.
• W2372207620 hasRelatedWork W2385964128 @default.
• W2372207620 hasRelatedWork W2388664705 @default.
• W2372207620 hasRelatedWork W2390607465 @default.
• W2372207620 hasRelatedWork W2391498659 @default.
• W2372207620 hasRelatedWork W2407885401 @default.
• W2372207620 hasRelatedWork W2414319492 @default.
• W2372207620 hasRelatedWork W2999829305 @default.
• W2372207620 hasRelatedWork W3031980681 @default.
• W2372207620 hasRelatedWork W3032710935 @default.
• W2372207620 hasRelatedWork W3032817873 @default.
• W2372207620 hasVolume "86" @default.
• W2372207620 isParatext "false" @default.
• W2372207620 isRetracted "false" @default.
• W2372207620 magId "2372207620" @default.
• W2372207620 workType "article" @default.