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- W2372247908 abstract "The cultural temperatures had much effect on docosahexaenoic acid (DHA) content in total lipid extracted from dried cell with different superoxide dismutase (SOD) expression levels. The growth and fatty acid composition of Schizochytrium sp. FJU-512 at different cultural temperatures were determined by dried cell and GC-MS. The expression level of SOD mRNA at different temperatures was compared by fluorescence quantitative-PCR. The results showed that low temperature was beneficial to DHA biosynthesis in marine Schizochytrium and the SOD mRNA increased to maximum at low temperature. The mechanism of Schizochytrium adapting to low temperature stress may be that the low temperature made oxygen easy to dissolve in cultural medium enhancing SOD expression that could improve the removal of reactive oxygen species. Unsaturated fatty acids were accumulated to maintain the mobility of membrane under the condition of low temperature. SOD gene was screened in EST library based on cDNAs extracted from cells of strains Schizochytrium sp. FJU-512 highly produces DHA. The amino sequence of SOD was analyzed by bioinformatics." @default.
- W2372247908 created "2016-06-24" @default.
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- W2372247908 date "2010-01-01" @default.
- W2372247908 modified "2023-09-23" @default.
- W2372247908 title "Effects on Docosahexaenoic Acid Biosynthesis and Expression of Superoxide Dismutase in Schizochytrium at Low Temperature" @default.
- W2372247908 hasPublicationYear "2010" @default.
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