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- W2372257547 abstract "To investigate the inheritance of high oleic acid content of W-4,a transgenic line of Brassica napus L.transformed with a ihpRNAi vector targeting the delta-12 oleate desaturase(fad2) gene,the generations derived from W-4(P1)×Westar(wild type,P2) including F1(RF1),F2(RF2),B1(RB1),B2(RB2) were collected.Oleic acid contents of the self-pollinated seeds harvested from parents and derived generations were analyzed by gas chromatography,and the plant resistances of parents and derived generations to kanamycin(Kan) were investigated at seedling stage.Results showed that the oleic acid contents in the seeds of P1 and P2 were(85.10±0.73)% and(62.16±3.68)%,respectively,and those of F1 and RF1 were(77.55±1.30)% and(77.34±2.46)%,respectively,which were high(≥69.5%).The seeds of B1 and RB1 all had high contents of oleic acid(≥69.5%).The seeds from the populations of B2 and RB2 gave a segregation ratio of 1∶1(χ2=1.0,χ2=0.16)for high oleic acid(≥69.5%)∶low oleic acid(69.5%) content,and those of F2 and RF2 gave a segregation ratio of 3∶1(χ2=0.96,χ2=0.11).All these suggested that the oleic acid content of W-4 was controlled by one pair of dominant gene and inherited in the mode of Mendelian.There was no cytoplasmic effect on the oleic content in this transgenic high oleic cross in Brassica napus L.In addition,the plants of P1,F1,RF1,B1,and RB1 exhibited kanamycin-resistant whereas the plants of P2 were sensitive to kanamycin.And the ratios of kanamycin-resistant to kanamycin-sensitive for the plants of B2 and RB2 were 1∶1 and 3∶1 for F2 and RF2.These indicated that the resistance of P1 to kanamicin was controlled by one pair of dominant gene." @default.
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- W2372257547 date "2013-01-01" @default.
- W2372257547 modified "2023-09-25" @default.
- W2372257547 title "Inheritance of high oleic acid trait in the transgenic line W-4 of Brassica napus" @default.
- W2372257547 hasPublicationYear "2013" @default.
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