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- W2372289969 abstract "Taking O,O-dimethyl organophosphorus pesticides as targets,a series of haptens were designed and synthesized. Four antibodies with broad-specificity to O,O-dimethyl organophosphorus pesticides were produced. Results showed that the broad specificity of antibody produced by hapten with unsaturated alkane arm was better than that of the haptens with amide bond on the benzene ring. The sensitivity of ELISA to O,O-dimethyl organophosphorus pesticides could be significantly improved by using the coating antigen with a characteristic substructure moiety of the targets. Under optimized conditions,eight O,O-dimethyl organophosphorus pesticides were detected using indirect competitive ELISA. The limits of detection ( LODs) are lower than the MRLS standard requirements with the LOD ranging from 2. 6 to 104 μg/kg. The recoveries of vegetable samples match the level of gas chromatography with the average recovery of 73. 9% 121. 4% and average RSD 10. 6% 18. 4% for lettuce,average recovery of 80. 4% 121. 2% and average RSD 13. 5% 24. 4% for pakchoi." @default.
- W2372289969 created "2016-06-24" @default.
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- W2372289969 date "2010-01-01" @default.
- W2372289969 modified "2023-09-25" @default.
- W2372289969 title "Development of Broad-specificity Immunoassay for Detection of O,O-Dimethyl Organophosphorus Pesticides" @default.
- W2372289969 hasPublicationYear "2010" @default.
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