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- W2372315795 abstract "In order to accurate detection of brucella from animals,three strains of monoclonal antibodies(MAbs)against Brucella,which was named as 5C10,4D2,and 3E4,were obtained by using hybridoma technique.With one of the MAbs 5C10,a competitive enzyme-linked immunosorbent assay(cELISA)for the serological detection of brucellosis was developed.Three hundred and twenty bovine and 147 ovine sera were used for the comparison of the detection effect of cELISA and the commercial kit.From the result,the agreement rates of the cELISA with the commercial kit were 95.6%and 92.5%for the bovine and ovine sera,respectively.The discrepant samples(25)were confirmed by the complement fixation test(CFT).We found that coincidence rate of the developed cELISA with CFT was higher(21/25)than the commercial kit(4/25).These results suggested that our cELISA for serological detection is more specific and sensitive than the commercial kit.It will be very useful in eradication program for brucellosis." @default.
- W2372315795 created "2016-06-24" @default.
- W2372315795 creator A5085512975 @default.
- W2372315795 date "2014-01-01" @default.
- W2372315795 modified "2023-09-26" @default.
- W2372315795 title "Development of monoclonal antibodies to Brucellaand validation of competitive enzyme-linked immunosorbent assay" @default.
- W2372315795 hasPublicationYear "2014" @default.
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