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- W2372365517 abstract "To truncate HIV 1 trans membrane protein gp41 and express the truncated gp41(tgp41) in E.coli. The trans membrane protein gp41 was truncated by PCR, the purified PCR products was ligated to cloning vector pGEM T, then the pGEM T tgp41 was digested with EcoRⅠand Sal Ⅰ , the tgp41 fragment was isolated and inserted to the corresponding restriction site on expression vector pGEX 4T 3. The recombinant plasmid pGEX 4T 3 tgp41 was transformed to host BL21(DE3), then expressed by adding IPTG. The truncated gp41 can be expressed in E.coli. and easy to be purified with affinity chromatography. It laid a foundation of further development of trans membrane protein." @default.
- W2372365517 created "2016-06-24" @default.
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- W2372365517 date "2003-01-01" @default.
- W2372365517 modified "2023-09-25" @default.
- W2372365517 title "Truncation and expression of HIV-1 trans-membrane protein gp41" @default.
- W2372365517 hasPublicationYear "2003" @default.
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