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- W2372377887 abstract "In order to develop a method to detect the presence of nucleic acids in Schistsoma japonicum, specific primers were designed based on the genome of S.japonicum and then PCR assay was used to detect DNAs from samples of eggs, adult worms, liver feces and peripheral blood of rabbits infected with S.japonicum. Meanwhile, the template DNAs of eggs and feces were diluted before amplification in order to test the sensitivity of the PCR assay. A 230 bp DNA fragment was obtained from all the samples being tested, and then the gene product was sequenced and matched in GenBank. It was confirmed that this product was a fragment of S.japonicum gene. Result in the PCR amplification sensitivity testing revealed that 0.021 egg DNA template would be enough to amplify this specific positive band. It is evident form the above that the detection of S.japonicum DNA by PCR exhibits higher sensitivity, especially to detect the specific DNAs from serum of hosts infected with S.japonicum, thus providing potential diagnostic values." @default.
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- W2372377887 date "2007-01-01" @default.
- W2372377887 modified "2023-09-27" @default.
- W2372377887 title "Detection of Schistosoma japonicum DNA by polymerase chain reaction" @default.
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