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- W2372381651 abstract "Objective: To isolate and purify ductal cells of submandibular glands (SMG) in vitro. Methods: The ductal cells of SD rats were isolated with density-gradient centrifugation under aseptic condition. Immunohistochemistry staining was used to determine the cells. The level of isolation and purification was evaluated by assay of amylase activity. Results: The cells in upper zone were positively stained with Anti-Pan cytokeratin and Anti-EMA,which were ductal specific antibodies. The activity of amylase of cells in upper and lower zones were 6% and 72% respectively. Conclusion: Pure ductal cells could be obtained with density-gradient centrifugation." @default.
- W2372381651 created "2016-06-24" @default.
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- W2372381651 date "2004-01-01" @default.
- W2372381651 modified "2023-09-26" @default.
- W2372381651 title "Isolation and purification of ductal cells of submandibular glands in vitro" @default.
- W2372381651 hasPublicationYear "2004" @default.
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