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- W2372447735 abstract "The gene encoding P15 of Cryptosporidium parvum(C.parvum) was amplified by PCR,and then inserted into the BamHⅠ site of pUC19 vector.Nucleotide sequencing showed that the gene was a 479 bp fragment and encoded a protein composed of 148 amino acid.The P15 gene was ligated into the BamHⅠ site of the expression vector pGEMEX-2,and then expressed in E.coli JM109(DE3) and mammal cells.SDS-PAGE and Western blot analyses showed that the P15 gene of C.parvum was expressed in E.coli and the molecular weight of the fusion protein was estimated as (50 000);the protein expressed in the mammal cells had a similar molecular weight((18 000)-(22 000)) with the native P15 of C.parvum." @default.
- W2372447735 created "2016-06-24" @default.
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- W2372447735 date "2005-01-01" @default.
- W2372447735 modified "2023-09-27" @default.
- W2372447735 title "Cloning and Expressing of the Gene Encoding P15 of Cryptosporidium parvum" @default.
- W2372447735 hasPublicationYear "2005" @default.
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