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- W2372450475 abstract "Objective: To optimize enzymatic extraction process of Cuscutae Semen,in order to increase extracting rates of hyperin and total flavonoids. Method: With the content of hyperin as index,effects of enzyme species,enzyme dosage,enzymolysis time,p H and temperature on enzymatic extraction process were investigated by single factor test. With composite score of extracting amounts of hyperin and total flavonoids as index,orthogonal test was used to optimize enzymatic extraction process with enzyme dosage, enzymolysis p H and temperature as factors. HPLC was employed to determine the content of hyperin with mobile phase of acetonitrile-0. 1% phosphoric acid solution( 17∶ 83) and detection wavelength at 360 nm. Result: Cellulase was selected,its optimum extraction technology was as follows: enzyme dosage 2. 0%,enzymolysis time of 2 hours, enzymatic hydrolysis of p H 4. 0,enzymolysis temperature at 45 ℃; extracting amounts of hyperin and total flavonoids were1. 408,26. 966 mg·g- 1,respectively. Conclusion: This optimized extracting process is stable,reasonable and applicable to industrial production of Cuscutae Semen." @default.
- W2372450475 created "2016-06-24" @default.
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- W2372450475 date "2014-01-01" @default.
- W2372450475 modified "2023-09-25" @default.
- W2372450475 title "Optimization of Enzymatic Extraction Technology of Cuscutae Semen" @default.
- W2372450475 hasPublicationYear "2014" @default.
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