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- W2372452029 abstract "the main aim of the present work is to observe the expression of heat shock protein 90(HSP90)in hypoxic rat myocardial cells.Also elucidated is the effect of HSP90 on the early hypoxia induced cardiac muscle injury.Methods: SD fetal rat myocardial cells were cultured in vitro,stochastically divided into three groups:normal control,hypoxia group and hypoxia group pretreated by geldanamycin(GA-added hypoxia group).Samples of myocardial cells were taken for the determination of HSP90 expression by Western blot analysis and indirect immunofluorescence at 1,3,6,12,24h after hypoxia. Changes of CK-MB and LDH content were detected.Results:expression level of HSP90 increased in rat myocardial cells endochylema after 3h of hypoxic injury,and the level reached highest at 12h after hypoxia.Compared to the hypoxia group,the level of LDH,CK-MB in GA-added hypoxia group is significantly higher at every point.Conclusion:hypoxia injury causes an increased expression of HSP90 in rat myocardial cells,and HSP90 may have endogenous anti-injury effect on early hypoxia injury." @default.
- W2372452029 created "2016-06-24" @default.
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- W2372452029 date "2007-01-01" @default.
- W2372452029 modified "2023-09-23" @default.
- W2372452029 title "The research of heat shock protein 90's expression in hypoxic rat myocardial cells" @default.
- W2372452029 hasPublicationYear "2007" @default.
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