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- W2372462004 abstract "A strain screened from the waste water of an oilfield can produce insoluble extracellular polysaccharide used in selective plugging which is instrumental in microbially enhanced oil recovery. However this strain is sensitive to high temperature and produces little extracellular polyssacharides above 40℃.Therefore pET-ecoRRF, pET-tteRRF and corresponding control plasmids were transformed into the strain. The new strain can grow in 45℃ .and western-blotindicates that the RRF genes have laeen expressed noticeably in the transgene strains. It has been showed that the transgenestrains are able to bear higher temperature. and the sustainable temperature is higher five to eight degrees than the originalstrain, Because Euternbacter sp. is related to Esacherichici coli the expression of RRF is better than the gene of Tlmnuusther-nwphilu.s in the strain of JD. At last it is successful to get a genetic engineering microbe which can endure the temperatureas high as 45℃ and produce extracellular polysaccharides." @default.
- W2372462004 created "2016-06-24" @default.
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- W2372462004 date "2007-01-01" @default.
- W2372462004 modified "2023-09-25" @default.
- W2372462004 title "Construction of a Genetic Engineering Strain That Can Produce Extracellular Polyssacharidesin Higher Temperature for MEOR" @default.
- W2372462004 hasPublicationYear "2007" @default.
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