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- W2372462022 abstract "Objective:To investigate the modulatory effect of IL-29 on trypsin-induced protease activated receptors(PARs) expression on P815 mast cell. Methods: After P815 mast cells were challenged with different concentrations of IL-29 alone or combined with trypsin for 2 h,6 h and 16 h,the challenged cells were collected and analysed by flow cytometry to detect the protein expression of PARs on P815 cells,and analysed by real time RT-PCR to detect the mRNA expression of PARs on P815 cells. Results: Compared with the corresponding control,IL-29 induced significantly decreased expression of PAR-1 at protein and mRNA level on P815 cells, and upregulated PAR-3,PAR-4 mRNA level on P815 cells,whereas IL-29 did little effect on the expressions of PAR-2,3,4 at protein level on P815 cells accordingly. Preincubation of mast cell with IL-29 did not alter trypsin-induced PAR-1 expression on P815 cells, whereas up-regulated expression of PAR-2,3,4 were detected when P815 cell were pre-treated with IL-29 before being challenged with trypsin compared with the corresponding control. Conclusion: IL-29 can upregulate trypsin-induced PARs expression on mast cells through which participated in mast cell related inflammation." @default.
- W2372462022 created "2016-06-24" @default.
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- W2372462022 date "2014-01-01" @default.
- W2372462022 modified "2023-09-23" @default.
- W2372462022 title "Effect of IL-29 on trypsin-induced protease-activated receptor expression on P815 mast cells" @default.
- W2372462022 hasPublicationYear "2014" @default.
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