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- W2372462978 abstract "Objectives To evaluate typing value by ribotyping technique for detection DNA of group B streptococcus( GBS) strains, to obtain the genotypes and genetic characteristics. MethodsThirty four GBS strains isolated from vaginas of asymptomatic pregnant women in Beijing were obtained.Ribotyping was carried out using the fragment of 16SrRNA as probe. The presence of bac, bca, ScpB, GlnA , and ScaA genes was detected in the genome of GBS strains by hybridization. Results All Strains contained genes of ScpB, ScaA, GlnA ; Strains belonging to serotype Ⅱ、Ⅱ/C have bca and bac genes; Most(60%) of strains belonging to Ia bac dorit have and bca genes.Strains expressing the serotype Ⅲ、Ⅲ/R lack of bca and bac genes. Only 3 different HindⅢ patterns, 7 and 10 different EcoRI and PvuII patterns among 34 strains, respectively; with the combination of three restriction endonucleases, a total 16 distinct ribotypes were obtained among 34 isolates; Combined analysis of all of our data revealed 22 distinct clonal genotypes. Conclusion Ribotyping genetic typing system should be useful for molecular epidemiological study of GBS; The combination of more molecular typing methods has potential value in the epidemiological study." @default.
- W2372462978 created "2016-06-24" @default.
- W2372462978 creator A5083319908 @default.
- W2372462978 date "2001-01-01" @default.
- W2372462978 modified "2023-09-25" @default.
- W2372462978 title "Genetic Characteristics of Group B Streptococcus by Southern Blot and Ribotyping" @default.
- W2372462978 hasPublicationYear "2001" @default.
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