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- W2372470275 abstract "Objective To explore how to extract and detect DNA from burned bone.Methods 30 adult femoral bone were incinerated at 200℃ 1 hour in normal pressure,and then freezed and grinded to be bone meal in the liquid nitrogen before decalcification by ethylenediamine tetraacetic acid(EDTA).The deoxyribonucleic acid(DNA) was extracted by improved phenol-chloroform,and the luminometer was used to detect the concentration and purity of the purified DNA.Subsequently DNA was five-fluorescent labeled,amplified by PCR,followed by capillary electrophoresis and the data analysis.Results DNA concentration was(28.5±1.7) ng/μl under the improved phenol-chloroform method,and the 16 genetic locus were detected,with the relative fluorescence units(RFU) of more than 500.The DNA concentration was about(4.7±0.8) ng /μl,and some gene cound not be detected under the traditional phenol-chloroform extraction.Conclusions The DNA extraction and detection can be performed in burned bone by the improved phenol-chloroform method,multiplex polymerase chain reaction,and gene locus analysis." @default.
- W2372470275 created "2016-06-24" @default.
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- W2372470275 date "2012-01-01" @default.
- W2372470275 modified "2023-09-23" @default.
- W2372470275 title "Experimental study of DNA extraction and detection in burned bone" @default.
- W2372470275 hasPublicationYear "2012" @default.
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