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- W2372506534 abstract "AIM:To explore the regulatory effect of microRNA-3666(miR-3666) on the expression of its target gene phosphatase and tensin homologue deleted on chromosome ten(PTEN) in leukemic cells.METHODS:miR-3666 expression levels in normal peripheral blood mononuclear cells and leukemic cells were determined by quantitative real-time PCR.miR-3666 targeting PTEN 3'-untranslated region(3'UTR) was predicted by TargetScan software.3'UTR of PTEN was inserted in the dual luciferase reporter vector psiCHECK2.The reporter activity was evaluated by the Dual-Luciferase Reporter Assay System after the luciferase promoter vector and miRNA were co-transfected into HEK293T cell line.K562 cells were transfected with synthetic miR-3666 inhibitor(anti-miR-3666) or a synthetic control miRNA(anti-miRC).The expression of PTEN protein in the above transfected K562 cells was determined by Western blotting.RESULTS:miR-3666 was up-regulated in the human leukemic cell lines and primary leukemic cells compared to normal peripheral blood mononuclear cells.The results of dual luciferase assays validated PTEN as a specific target gene of miR-3666.Inhibition of miR-3666 resulted in an up-regulation of PTEN protein expression in the K562 cells.CONCLUSION:miR-3666 is over-expressed in leukemic cells.The abnormal over-expression of miR-3666 may play a key role in leukemia due to the down-regulation of PTEN." @default.
- W2372506534 created "2016-06-24" @default.
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- W2372506534 date "2014-01-01" @default.
- W2372506534 modified "2023-09-25" @default.
- W2372506534 title "MicroRNA-3666 regulates expression of phosphatase and tensin homologue deleted on chromosome ten in leukemic cells" @default.
- W2372506534 hasPublicationYear "2014" @default.
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