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- W2372524804 abstract "Objective:To investigate the different killing effects on lymphoma Raji cells with cytosine deaminase (CD) and herpes simplex virus thymidine kinase (HSV tk) double suicide genes coexpressed compared with single gene mediated by retrovirus. To find a more efficient and low toxicity suicide gene therapy for lymphoma.Methods:CD and HSV tk double suicide genes were transfected into PA317 cells using lipofectamine.The positive clones were picked out and cultured after G418 selected.The viral supernatant was collected and concentrated.The lymphoma cells were infected with the concentrated virus containing the double suicide genes.After G418 selection, RT PCR was resorted to demonstrate the successful transcription of CD and HSV tk genes.The Raji/CD + tk and Raji cells in culture were respectively treated with 5 FC and/or GCV.The cytoxicity efficacy was evaluated by microculture tetrajolium test (MTT) method.Results:The virus containing double suicide gene was produced in PA317 cells.Double suicide genes were stably and efficiently expressed in Raji cells after being infected with the provirus.Exposure to the matching prodrugs (GCV and 5 FC) showed decreased cell growth rate (13.83 %) compared with exposure to 5 FC (57.68 %) or GCV (50.65 %) alone.There are remarkably significant between the groups.Conclusion:The double suicide gene system mediated by retroviral vector enhanced killing effect remarkably on lymphnoma cells than CD/5 FC or tk/GCV system alone.The killing effect of combination 5 FC with GCV on Raji/CD + tk cells is more effective than that of using 5 FC or GCV alone. The CD + tk /5 FC+GCV co expression system is more effective for killing effect on lymphoma cells than that of CD/SFC or TK/GCV system alone." @default.
- W2372524804 created "2016-06-24" @default.
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- W2372524804 date "2003-01-01" @default.
- W2372524804 modified "2023-09-24" @default.
- W2372524804 title "ENHANCED KILLING EFFECT OF DOUBLE SUICIDE GENES TRANSFERRED INTO Raji LYMPHOMA CELLS BY RETROVIRAL VECTOR" @default.
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