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- W2372543938 abstract "This study aimed to provide pure NS3 protein as antigen for ELISA,which could distinguish BVDV vaccinated from infected cattle.A pair of primers were designed according to the major antigenic region of bovine virus diarrhea NS3 gene derived form GenBank.The NS3 gene with a size of 1 206bp was amplified by PCR and subcloned in to prokaryotic vector pET-32a.The recombinant plasmid pET-32a-NS3 was transformed in to E.coli Transetta(DE3)and then expressed by IPTG induction.The NS3 recombinant protein was analyzed by SDS-PAGE and Western blot.The result showed that the NS3 recombinant protein was mainly expressed in the form of inclusion body and the molecular weight of the fusion protein was about 61ku.The result of the Western blot showed that the NS3 recombinant protein could be specifically recognized by positive serum to BVDV,and may be used as the coating antigen to develop a diagnosis kit against BVDV." @default.
- W2372543938 created "2016-06-24" @default.
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- W2372543938 date "2014-01-01" @default.
- W2372543938 modified "2023-09-23" @default.
- W2372543938 title "Prokaryotic Expression and Identification of the NS3 Protein of Bovine Viral Diarrhea Virus" @default.
- W2372543938 hasPublicationYear "2014" @default.
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