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- W2372552181 abstract "The plasmid pTrc harboring Glutathione synthetases gene (gsh I and gsh II) was transformed into different E. coli host strains in order to screen the best one. E. coli BL21 (pTrc gsh) can express the related enzymes with high level and stable activity. Two step culture was developed for the enzymes production. The engineering strain was incubated at 37°C and pH7.2 up to about OD 550 =0.5 during the first step. The condition was then switched to 34°C and pH6.7 after induction with IPTG(0.1mmol/L). The enzyme activity of the dry cell reached 114.9μg/(mg·min). The optimum temperature and pH of the enzyme activity were 37°C and pH6.7 respectively." @default.
- W2372552181 created "2016-06-24" @default.
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- W2372552181 date "2000-01-01" @default.
- W2372552181 modified "2023-09-25" @default.
- W2372552181 title "Study on the Glutathione Synthetases Expression in Recombinant Escherichia coli and Properties" @default.
- W2372552181 hasPublicationYear "2000" @default.
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