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- W2372570003 abstract "Plasmid DNA(p301-bG1) used in transgenic research of familiar edible fungi was added to edible fungi samples as simulated positive samples.Total DNAs were extracted from 19 samples by CTAB method.Multiplex PCR detection method was developed for the target DNA fragments with the size of 165,398,545 and 600 bp of four transgenes,i.e.NOS,BAR,GUS and NPT-Ⅱ respectively.Five combinations of duplex PCR including NOS-GUS,BAR-GUS,NOS-BAR,NOS-NPT Ⅱ and BAR-NPTⅡ,as well as two combinations of triplex PCR including NOS-BAR-GUS and NOS-BAR-NPTⅡ,were performed." @default.
- W2372570003 created "2016-06-24" @default.
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- W2372570003 date "2005-01-01" @default.
- W2372570003 modified "2023-09-23" @default.
- W2372570003 title "Detection of genetically modified component in familiar edible fungi by multiplex PCR" @default.
- W2372570003 hasPublicationYear "2005" @default.
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