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- W2372581567 abstract "【Objective】The transgenic safflower was obtained to express human hyaluronidase gene(hPH20)and lay foundation for development of hPH20 products.【Method】The hPH20 gene was synthesized and NcoⅠ/Hind Ⅲ enzyme sites were introduced at both ends.Then the hPH20 gene was inserted into plant expression vector pOBT by NcoⅠ/HindⅢ.The recombinant plasmid was named pOBT-hPH20 and was transferred into Agrobacterium tumefaciens EHA105.The hPH20 gene was introduced into safflower via Agrobacterium-mediated method and positive transgenic plants were determined by PCR analysis.【Result】The recombinant plasmid pOBT-hPH20 was successfully constructed.PCR confirmed that hPH20 gene was integrated into the genome of safflower plant and three transgenic plants were obtained.【Conclusion】The safflower regeneration system was constructed successfully and hPH20 gene was successfully transformed into safflower plants." @default.
- W2372581567 created "2016-06-24" @default.
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- W2372581567 date "2015-01-01" @default.
- W2372581567 modified "2023-09-25" @default.
- W2372581567 title "Expression of human hyaluronidase gene in oil body of safflower" @default.
- W2372581567 hasPublicationYear "2015" @default.
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