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- W2372589403 abstract "Aim To test the specific gene expression of single mouse pre-implantation embryos by applying real-time fluorescence quantitative RT-PCR(rQ-RT-PCR).Methods The mouse oocytes and pronuclear-stage embryos(zygote) which are cultured in vitro to blastocysts were collected.Then,the rQ-RT-PCR was used to test quantitatively the expression of gene Tcl1 in the oocytes and 2-cell stage embryos.The same methods were applied in the examination of gene Tcl1 in mouse embyos in every stage before implantation.Results Tcll was found quantitatively in multiple oocytes,single oocyte and 2-cell stage embryos.The expression of Tcll in multiple oocytes and 2-cell embryos was much higher than that in single oocyte and 2-cell embryo.The method could be applied in every embryo stage.The highest expression of Tcll was found in oocyte,pronuclear stage embryos and 2-cell stage embryos,however,its expression in 4-cell and 8-cell embryos,morulas and blastocysts was extremely low.Conclusion The rQ-RT-PCR can be applied to quantitative examination of gene expression in single mouse pre-implantation embryo." @default.
- W2372589403 created "2016-06-24" @default.
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- W2372589403 date "2010-01-01" @default.
- W2372589403 modified "2023-09-23" @default.
- W2372589403 title "Applying real-time fluorescence quantitative RT-PCR to test the specific gene expression of single mouse pre-implantation embryos" @default.
- W2372589403 hasPublicationYear "2010" @default.
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