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- W2372608214 abstract "A pair of specific primers were designed according to the published 118 bp conserved sequence of polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) in GenBank and a serial dilutions of a recombinant plasmid were prepared and used to generate standard curves, to establish a real-time fluorescent quantitative PCR method for detection of BmCPV. The results showed that the linear relationship between virus concentration (X) and Ct (Y) was Y = -3. 582 lgX + 38.748 with the correlation coefficient R2 = 0999. The method was very sensitive, specific and reproducible. It can be applied in the rapid detection of BmCPV and the prevalence investigation of this disease." @default.
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- W2372608214 date "2013-01-01" @default.
- W2372608214 modified "2023-09-27" @default.
- W2372608214 title "[Development of real-time fluorescent quantitative PCR method for detecting Bombyx mori cytoplasmic polyhedrosis virus]." @default.
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