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- W2372609885 abstract "Objective To establish a rapid propagation system of Aconitum carmichaeli.MethodsWith tissue culture method,18,9,and 12 kinds of hormones combinations as media were developed to induce callus from different explants derived from A.carmichaeli,the differentiation of cluster buds,and the rooting of regenerated seedlings,respectively.Results For A.carmichaeli,the optimum culture medium for callus induction of stem-tips and stem segments was identified to be MS+NAA 0.2 mg/L+6-BA 2.0 mg/L+PVP(or AC)3.0 g/L.Optimum culture medium for callus induction on leaves was identified to be MS+NAA 0.2 mg/L+6-BA 4.0 mg/L+PVP(or AC)3.0 g/L.The optimum culture media for callus propagation and cluster buds induction were identified to be MS+NAA 0.2 mg/L+6-BA 2.5 mg/L+LH 200 mg/L+PVP 3.0 g/L and MS+NAA 0.1 mg/L+6-BA 2.0 mg/L+LH 200 mg/L+PVP 3.0 g/L.The optimum culture medium for rooting induction was identified to be MS+IBA 1 mg/L+AC 3.0 g/L.Conclusion Industrialized rapid propagation of seedling can be realized by in vitro tissue culture with different explants such as shoot tips,leaves,and stem segments with or without axillary bud." @default.
- W2372609885 created "2016-06-24" @default.
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- W2372609885 date "2007-01-01" @default.
- W2372609885 modified "2023-09-23" @default.
- W2372609885 title "In vitro culture and rapid propagation of Aconitum carmichaeli" @default.
- W2372609885 hasPublicationYear "2007" @default.
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