Matches in SemOpenAlex for { <https://semopenalex.org/work/W2372612874> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2372612874 abstract "Objective To construct the E.coli expressiom system of the HBc-IsdB50-285 fusion protein,and to explore the expression efficacy of the HBc-IsdB50-285 fusion protein in prokaryotic system.Methods HBc-IsdB50-285 fusion gene fragment was designed and cloned,and the expression vector pET-28-HBc-IsdB50-285 was construct and transformed into E.coli BL21,the expression of protein was inducted by IPTG.The solubility of protein and its relative molecular mass were analyzed by SDS-PAGE method;BCA assay was used to detect the level of protein;the immune activity of the recombinant protein was detected by immune inoculation method.40 mice were divided into rIsdB+adjuvant group(n=10),HBc-IsdB50-285+adjuvant group(n=10),HBc-IsdB50-285group(n=10),and PBS group(n=10).The values of special IgG of the mice were tested by ELISA method.The mice were immunized and attacked by Stathylococcus aureus Newman strain,and the immune protection rate of the recombinant protein was detected.Results The expression vector pET-28-HBc-IsdB50-285 was successfully constructed through identification of sequencing and restriction enzyme digestion.The SDS-PAGE results showed that the recombinant protein existed in a soluble form in the supernatant with relative molecular mass about 44 000.The level of purificated protein was 1.0g·L-1.The ELISA results showed that the value of antibody titer of the mice in HBcIsdB50-285+adjuvant group was lower than that in rIsdB +adjuvant group when the rIsdB were regarded as coating antigen(P0.01);the values of antibody titer of the mice in HBc-IsdB50-285+adjuvant group was higer than those in rIsdB +adjuvant group and HBc-IsdB50-285 group when the HBc-IsdB50-285 were regarded as coating antigen(P0.05);the attack test results showed that the protection rate of Staphylococcus aureus of the mice in HBC-IsdB50-285+adjuvant group was lower than that in rIsdB+adjuvant group(P0.05).Compared with HBc-IsdB50-285 group,the protection rate of Staphylococcus aureus of the mice in HBC-IsdB50-285 +adjuvant group had no significant difference(P0.05).Compared with PBS group,the protection rates of Staphylococcus aureus of the mice in HBcIsdB50-285+adjuvant group(P 0.01)and HBc-IsdB50-285group(P 0.05)were increased.Conclusion A prokaryotic expression vector of HBC-IsdB50-285 is constructed,and the HBC-IsdB50-285 fusion protein with better biological activities is expressed in E.coli BL21 successfully." @default.
- W2372612874 created "2016-06-24" @default.
- W2372612874 creator A5086549725 @default.
- W2372612874 date "2015-01-01" @default.
- W2372612874 modified "2023-09-25" @default.
- W2372612874 title "Fusion expression of Staphylococcus aureus IsdB epitope protein and HBc protein" @default.
- W2372612874 hasPublicationYear "2015" @default.
- W2372612874 type Work @default.
- W2372612874 sameAs 2372612874 @default.
- W2372612874 citedByCount "0" @default.
- W2372612874 crossrefType "journal-article" @default.
- W2372612874 hasAuthorship W2372612874A5086549725 @default.
- W2372612874 hasConcept C104317684 @default.
- W2372612874 hasConcept C123894998 @default.
- W2372612874 hasConcept C147483822 @default.
- W2372612874 hasConcept C153911025 @default.
- W2372612874 hasConcept C159047783 @default.
- W2372612874 hasConcept C159654299 @default.
- W2372612874 hasConcept C185592680 @default.
- W2372612874 hasConcept C195616568 @default.
- W2372612874 hasConcept C203014093 @default.
- W2372612874 hasConcept C2777863537 @default.
- W2372612874 hasConcept C2779489039 @default.
- W2372612874 hasConcept C32611913 @default.
- W2372612874 hasConcept C33161422 @default.
- W2372612874 hasConcept C40767141 @default.
- W2372612874 hasConcept C523546767 @default.
- W2372612874 hasConcept C54355233 @default.
- W2372612874 hasConcept C55493867 @default.
- W2372612874 hasConcept C86803240 @default.
- W2372612874 hasConcept C8891405 @default.
- W2372612874 hasConcept C89423630 @default.
- W2372612874 hasConceptScore W2372612874C104317684 @default.
- W2372612874 hasConceptScore W2372612874C123894998 @default.
- W2372612874 hasConceptScore W2372612874C147483822 @default.
- W2372612874 hasConceptScore W2372612874C153911025 @default.
- W2372612874 hasConceptScore W2372612874C159047783 @default.
- W2372612874 hasConceptScore W2372612874C159654299 @default.
- W2372612874 hasConceptScore W2372612874C185592680 @default.
- W2372612874 hasConceptScore W2372612874C195616568 @default.
- W2372612874 hasConceptScore W2372612874C203014093 @default.
- W2372612874 hasConceptScore W2372612874C2777863537 @default.
- W2372612874 hasConceptScore W2372612874C2779489039 @default.
- W2372612874 hasConceptScore W2372612874C32611913 @default.
- W2372612874 hasConceptScore W2372612874C33161422 @default.
- W2372612874 hasConceptScore W2372612874C40767141 @default.
- W2372612874 hasConceptScore W2372612874C523546767 @default.
- W2372612874 hasConceptScore W2372612874C54355233 @default.
- W2372612874 hasConceptScore W2372612874C55493867 @default.
- W2372612874 hasConceptScore W2372612874C86803240 @default.
- W2372612874 hasConceptScore W2372612874C8891405 @default.
- W2372612874 hasConceptScore W2372612874C89423630 @default.
- W2372612874 hasLocation W23726128741 @default.
- W2372612874 hasOpenAccess W2372612874 @default.
- W2372612874 hasPrimaryLocation W23726128741 @default.
- W2372612874 hasRelatedWork W1772367847 @default.
- W2372612874 hasRelatedWork W2342304923 @default.
- W2372612874 hasRelatedWork W2405576511 @default.
- W2372612874 hasRelatedWork W2415584352 @default.
- W2372612874 hasRelatedWork W2436556395 @default.
- W2372612874 hasRelatedWork W2468571725 @default.
- W2372612874 hasRelatedWork W2989693066 @default.
- W2372612874 hasRelatedWork W3015335201 @default.
- W2372612874 hasRelatedWork W3028881490 @default.
- W2372612874 hasRelatedWork W3029340966 @default.
- W2372612874 hasRelatedWork W3029552490 @default.
- W2372612874 hasRelatedWork W3029619588 @default.
- W2372612874 hasRelatedWork W3029968364 @default.
- W2372612874 hasRelatedWork W3030461537 @default.
- W2372612874 hasRelatedWork W3031752640 @default.
- W2372612874 hasRelatedWork W3031780487 @default.
- W2372612874 hasRelatedWork W3032537110 @default.
- W2372612874 hasRelatedWork W3141666274 @default.
- W2372612874 hasRelatedWork W3146107517 @default.
- W2372612874 hasRelatedWork W66749156 @default.
- W2372612874 isParatext "false" @default.
- W2372612874 isRetracted "false" @default.
- W2372612874 magId "2372612874" @default.
- W2372612874 workType "article" @default.