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- W2372734314 abstract "AIM: To establish the model of Plasmodium berghei (P. berghei) transfected with Plasmodium falciparum (P. falciparum) apical membrane antigen-1 (AMA-1) gene. METHODS: The transfection plasmid pD_B.D_ Tm .D_B./A_B.A_F.D_B. containing P.falciparum AMA-1 was enzymed by BglI. Blood stages of P.berghei were cultured in vitro,and mature schizonts were purified. Parasites transfected by electroporation were injected into tail veins of mice. Transfected parasites were selected by treating mice with pyrimethamine. DNA of transfected,resistant parasites was isolated and analyzed by PCR.RESULTS: The gene encoding P.falciparum AMA-1 was amplified by PCR in the transfected parasites. CONCLUSION: The model of P.berghei transfected with P.falciparum AMA-1 gene was established,which provided a basis for further study." @default.
- W2372734314 created "2016-06-24" @default.
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- W2372734314 date "2006-01-01" @default.
- W2372734314 modified "2023-09-24" @default.
- W2372734314 title "Establishment of model of Plasmodium berghei transfected with Plasmodium falciparum apical membrane antigen-1 gene" @default.
- W2372734314 hasPublicationYear "2006" @default.
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