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- W2372804538 abstract "AIM:To explore the mutual effect of co-culture of wild-type astrocytes (ASC) and motor neurons VSC4.1 (VSC) on the respective ability to produce reactive oxygen species (ROS). METHODS:The inhibition rates of cell growth in ASC and VSC in co-culture or independent culture was detected after exposed to excitatory stimulus by MTT method. Real-time observation of ROS production by ASC and VSC labeled with Hoechst 33342 was detected by confocal microscopy under the conditions of co-culture or independent culture. RESULTS:Higher concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in ASC alone,while lower concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in VSC only. Real-time observation by confocal microscopy showed that ROS production by VSC under the condition of co-culture,which showed a notable increase at 15 min,was significant less than that in independent culture,which peaked at 5 min and was gradually decreased. ROS production by ASC in co-culture began to increase significantly at 10 min. CONCLUSION:Compared to independent culture,ASC reduces the resting ROS production by co-cultured with VSC,while ASC prolongs the duration of ROS production by VSC after exposed to excitatory stimulus." @default.
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- W2372804538 date "2009-01-01" @default.
- W2372804538 modified "2023-09-23" @default.
- W2372804538 title "Change of reactive oxygen species production in co-culture model of rat astrocytes and motor neurons VSC 4.1" @default.
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