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- W2372831812 abstract "Research of new genes underlying embryonic development is important for the molecular mechanism.A cDNA fragment was cloned from a cDNA library of mouse,and homology with gene 2810025M15Rik available in Genbank database.In this paper,the expression pattern and the methylation analysis of CpG sites upstream the promoter of this novel gene were reported.In this paper,2810025M15Rik expression patterns were char acterized by real-time quantitative RT-PCR and in situ hybridization;the methylation analysis of the CpG island was detected with Bisulfite Sequencing.Introduced the expression pattern of the gene at embryonic period.A persistent expression was detected throughout the embryonic development and in some others important tissues by real-time quantitative RT-PCR and in situ hybridization.A higher expression level of the 2810025M15Rik mRNA was observed in the brain and branchial arches,fore-limb,tail bud by whole mount in situ hybridization analysis during earlier stage of mouse(E10.5 and E11.5).Furthermore,bisulfite sequencing analysis of the CpGs upstream the promoter showed they were unmethylated.The results suggested that 2810025M15Rik might be implicated in developmental functions,particularly during earlier development stage of mouse." @default.
- W2372831812 created "2016-06-24" @default.
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- W2372831812 date "2011-01-01" @default.
- W2372831812 modified "2023-09-25" @default.
- W2372831812 title "Expression of 2810025M15Rik gene during mouse embryonic development" @default.
- W2372831812 hasPublicationYear "2011" @default.
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