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- W2372879503 abstract "Cryptosporidium baileyi oocysts were purified from feces with discontinuous sugar concentration gradient,treated by freeze-thawed,sonicated and centrifugation.The purified oocyst antigen of C.baileyi was used to immunize hens that were producing eggs.The high titer egg yolk antibody was produced post sixth time immunized.Horseradish peroxidase(HRP) was used to label rabbit anti-chicken IgG.The working concentration of HRP-labelled rabbit anti-chicken IgG was 1∶4 000~1∶6 000.An optimal coating concentration of C.baileyi antigen was 8.15 mg·L-1 and the coating condition was 4 ℃ staying overnight,using 1×106 mg·L-1 BSA-PBST to dilute egg yolk." @default.
- W2372879503 created "2016-06-24" @default.
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- W2372879503 date "2009-01-01" @default.
- W2372879503 modified "2023-09-26" @default.
- W2372879503 title "Development of indirect ELISA for the detection of Cryptosporidium baileyi specific IgY" @default.
- W2372879503 hasPublicationYear "2009" @default.
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