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- W2372897929 abstract "Objective To found sensitive and reliable method to detect trophoblastic tumor cells from peripheral blood, establish experimental method for early diagnosis of metastasis of gestation trophoblastic tumor (GTT). Methods Known numbers of JAR cells were mixed into 10ml Peripheral blood as a model for trophoblastic tumor cells in the peripheral blood. Quantitative detection of β-hcG mRNA expression of JAR cells by fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCCR) were performed. Results FQ-RT-PCR could detect β-hCG mRNA equal to one JAR cell expression. It could be detected102 trophoblasts when mixed into maternal blood after enrichment. Conclusions The method used in this research proved very sensitive in detection of hCG-mRNA expression cells, and it would be used in detecting trophoblastic tumor cells and benefit finding early metastasis of GTT." @default.
- W2372897929 created "2016-06-24" @default.
- W2372897929 creator A5072524985 @default.
- W2372897929 date "2000-01-01" @default.
- W2372897929 modified "2023-09-24" @default.
- W2372897929 title "Study of fluorescence quantitative polymerase chain reaction in detection of JAR cells from peripheral blood" @default.
- W2372897929 hasPublicationYear "2000" @default.
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