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- W2372904223 abstract "Objective To observe the effect of disruption of hypoxia inducible factor-1α(HIF-1α) pathway by small hairpin RNA(shRNA) on chemosensitivity of human hepatocellular carcinoma(HCC) cells and to reveal the correlative mechanisms.Methods Plasmid of pshRNA-HIF-1α was transfected into HepG2 cells by lipofectamine.HepG2/pshRNA-HIF-1α(HepG2/pshRNA) cell lines were obtained by selection of HepG2 cells in G418.Meanwhile,plasmid of empty vector(pHK) was transfected as a control(HepG2/pHK).The mRNA and protein expression levels of HIF-1α and mdr1 were investigated by RT-PCR and Western blot respectively.Using CoCl2 to simulate the hypoxia condition,growth inhibition and apoptosis rates of HepG2 cells under different dosages of chemotherapeutic agents(adriamycin) were measured by MTT assay and flow cytometry(FCM).Results Compared with HepG2/pHK cells,the mRNA and protein expression levels of HIF-1α and mdr1 were obviously down-regulated in HepG2/pshRNA cells.At the same time,the proliferation inhibition and apoptosis rates were evidently increased after transfection with pshRNA-HIF-1α(P0.05),which decreased the expression of HIF-1α to 82.18% at mRNA level and 75.51% at protein level.There was no significant effect of transfection pHK(P0.05).Conclusion These data demonstrates that HIF-1α interference by shRNA increased the sensitivity of HCC chemotherapy and the reversal of multidrug resistance,which may be done by down-regulating the transcription of mdr1 and the translation of P-gp.Blocking HIF-1α in HCC cells may offer an new avenue for gene therapy." @default.
- W2372904223 created "2016-06-24" @default.
- W2372904223 creator A5062841037 @default.
- W2372904223 date "2008-01-01" @default.
- W2372904223 modified "2023-09-25" @default.
- W2372904223 title "Effect of Silencing of Hypoxia Inducible Factor-1 Alpha Gene on Chemosensitivity of Human Hepatocellular Carcinoma Cells and Correlative Mechanisms" @default.
- W2372904223 hasPublicationYear "2008" @default.
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