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- W2372912352 abstract "[Objective] The research aimed to study the effects of different factors on spindle of mouse MII oocytes,and provide references for the oocyte spindle.[Method] The mouse MII oocytes were treated with different conditions,and their spindles were observed by immunocytochemical method and laser scanning confocal microscopy.[Result] The spindles of oocytes were abnormal at 25 ℃ for 20 min,and disappeared at 25 ℃ for 30 min or at 4 ℃ for 10 min.5,10,20 and 40 μg/ml Cytochalasin B(CB) for 2 h had no effects on the spindles of the oocytes.The spindles of oocytes were abnormal in 20 μmol/L colchicines for 1 h,and disappeared in 40 μmol/L colchicines for 1 h.The structure of spindles were perfect for 1-12 h when the oocytes were fixed in 4% paraformaldehyde.[Conclusion] The spindle of the mouse MII oocytes is sensitive to environmental changes,and the control of environmental factors is crucial for normal function of the spindle in vitro manipulation." @default.
- W2372912352 created "2016-06-24" @default.
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- W2372912352 date "2008-01-01" @default.
- W2372912352 modified "2023-09-28" @default.
- W2372912352 title "Effects of Different Factors on Spindle of Mouse MII Oocytes" @default.
- W2372912352 hasPublicationYear "2008" @default.
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