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- W2372917892 abstract "Double-strand RNA (dsRNA) induced sequence-specific post-transcriptional gene silencing was known as RNA interference (RNAi). siRNA, an important intermediate of the RNA-interference pathway, are very effective in antiviral therapy in many organisms including mammals and plants. Oligonucleotides expressed siRNA were designed and synthesized according to the coat protein (CP) gene of Tobacco mosaic virus (TMV) and subcloned to the binary vector pBI121. The interference effects of siRNA targeting CP gene on TMV infection were studied by Agrobacterium-mediated transient expression system. Our results showed that transiently expressed siRNA could specifically interfere with TMV infection. Fourteen days after inoculation with TMV, the upper leaves of Nicotiana tobacum infiltrated with Agrobacterium tumefaciens cultures containing pBI121/siRNA were free of mosaic symptom. Northern blot assays also showed there were no or much less TMV RNA accumulation in these leaves. As for local lesion host,Nicotiana glutinosa, transiently expressed siRNA could reduce the lesion number resulting from TMV infection. In addition, transiently expressed siRNA targeting CP gene of TMV was unable to inhibit Cucumber mosaic virus (CMV), which indicated that the interference effect of siRNA was strictly dependent on the specific nucleotide sequence. Therefore, we supposed that the combination of siRNA technique and Agrobacterium-mediated transient expression system could be used as an antiviral treatment or rapid diagnostic tool in plants." @default.
- W2372917892 created "2016-06-24" @default.
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- W2372917892 date "2006-01-01" @default.
- W2372917892 modified "2023-09-23" @default.
- W2372917892 title "Transiently expressed siRNAs targeting coat protein gene of Tobacco mosaic virus interferes with the virus infection" @default.
- W2372917892 hasPublicationYear "2006" @default.
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