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- W2372956430 abstract "The electroporation parameters for Bacillus alcalophilus TCCC11263 were optimized and an electroporation system for B. alcalophilus TCCC11263 was developed. The parameters included cell growth stage, electric field strength, electric buffer constituents and plasmid concentration. The results showed that the transformation efficiency of 0.14 ×103 transformants per μg DNA was achieved when OD600 of the culture for B. alcalophilus TCCC11263 was 1.0. 0.5 mol sorbitol, 0.5mol mannitol and 10% glycerol were optimized as the constituents for the electric buffer. The electroporation efficiency was up to 1.5 ×103 transformants per μg DNA under electric field strength 21 kV/cm, electric resistance 200Ω and capacitance 25 μF when 0.7 μg DNA was applied." @default.
- W2372956430 created "2016-06-24" @default.
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- W2372956430 date "2010-01-01" @default.
- W2372956430 modified "2023-09-24" @default.
- W2372956430 title "Optimization of electroporation for Bacillus alcalophilus TCCC11263" @default.
- W2372956430 hasPublicationYear "2010" @default.
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