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- W2372965275 abstract "Aim:To compare the functional difference between two kinds of blood-brain -barrier(BBB)models in vitro.Methods:To establish two different BBBmodels by coculturing rat Type-I astrocytes(TIA)with rat brain microvascular endothelial cells(BMEC)and human umbilical vein cells(HUVEC)respectively on the two opposite surface of Transwell polyester membrane.And then to determine the apparent permeability coefficent(papp)of sodium fluorescent and 125 I-BSA and the activity of γ-glutamyl transpeptidase(γ-GT).Results:The Papp of FLU and 125 I-BSA across the BBB by BMEC and TIA are lower than that by HUVEC and TIA.The activity of γ-GT is 11.3±0.66U/mg in BMEC model.none in HUVEC model.Conclusion:The BBB model in vitro by coculturing allogenic endothelial cells with TIA is the best way to mimic the BBB in vivo." @default.
- W2372965275 created "2016-06-24" @default.
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- W2372965275 date "2003-01-01" @default.
- W2372965275 modified "2023-09-25" @default.
- W2372965275 title "Functional Comparison of Two kinds of Blood-Brain-Barrier Models in vitro" @default.
- W2372965275 hasPublicationYear "2003" @default.
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