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- W2372971569 abstract "Objective:To determinate the metabolites of quercetin in rat plasma after an oral administration by LC-MS.Methods:The rats were administrated quercetin monomer solution(100 mg·kg-1)by oral administration and the blood sample were prepared.Quercetin,isorhamnetin and tamarixetin were extracted from rat plasma after enzymatic hydrolysis using β-glucuronidase/sulfatase(G1512)in aqueous methanol.After enzymatic hydrolysis,the analytes of interest were separated on a Diamonsil C18 column.The mobile phase was acetonitrile-1% acetic acid(29∶71)with a flow rate of 1.0 mL·min-1.A Bruker mass spectrometry equipped with an atmospheric pressure electrospray ionization (APESI)source was used as detector and was operated in negative ion mode.Results:Quercetin,isorhamnetin and tamarixetin were identified through comparing with the standard spectrum and the results of mass spectrometry,i.e.m/z 300.9 was quercetin,m/z 314.9 was isorhamnetin and m/z 314.9 was tamarixetin.Conclusion:Enzymatic hydrolysis can be used to identify the quercetin metabolites in rat plasma.And this method can be a guidance for pharmacokinetics studies upon traditional Chinese medicine." @default.
- W2372971569 created "2016-06-24" @default.
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- W2372971569 date "2009-01-01" @default.
- W2372971569 modified "2023-09-22" @default.
- W2372971569 title "LC-MS determination of brief quercetin metabolites in rat plasma" @default.
- W2372971569 hasPublicationYear "2009" @default.
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