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- W2372987972 abstract "AIM: To establish a simple and reliable method for primary culture of mouse pulmonary microvascular endothelial cells(PMVECs),and to investigate the ability of angiogenesis of the cells in vitro.METHODS: PMVECs from C57BL/6 new born mice were cultured with peripheral pulmonary tissue pasted method.The cells were observed under inverted phase-contrast microscope and identified by immunocytochemical and immunofluorescent methods.PMVECs were seeded at the density of 20 000 cells per well in 96-well plate on top of growth factor-reduced matrigel and incubated for 24 h at 37 ℃ in 5% CO2.The plates were examined every 2 h for tube formation under an inverted microscope.RESULTS: The primary PMVECs showed fusiform shape or polygonal,regular cobblestone morphology after fusion to monolayer,and expressed factor Ⅷ-related antigen(ⅧF-Ag).Under fluorescence microscope,those cells demonstrated positive staining for uptaking acetylated low-density lipoprotein(DiI-Ac-LDL) and binding Bandeiraea simpli-cifolia lectin I(FITC-BSI).The purity of PMVECs was above 90%.PMVECs formed capillary-like structures within 4 h to 6 h after plated on matrigel and fully developed a honeycomb appearance at 10~12 h,and then the capillary tubes began to break apart.CONCLUSION: The tissue block pasted method for primary cultivation of PMVECs is easy to handle,with high yield and purity,and will further facilitate related studies of well-established tube-formation assay in vitro." @default.
- W2372987972 created "2016-06-24" @default.
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- W2372987972 date "2012-01-01" @default.
- W2372987972 modified "2023-09-23" @default.
- W2372987972 title "Primary culture,identification and in vitro angiogenesis of mouse pulmonary microvascular endothelial cells" @default.
- W2372987972 hasPublicationYear "2012" @default.
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