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- W2372994370 abstract "Canine parvovirus(CPV)encoded VP2 gene shows frequent genetic variation,which contributes to the tropism and pathogenicity changes of CPV.This study aimed to prepare the recombinant VP2protein(rVP2)encoded by a highly pathogenic CPV strain(NY strain)by prokaryotic expression system.A recombinant plasmid pET28a-CPV-VP2 was constructed and transfected into E.coli BL21(DE3),rVP2 was expressed at different temperatures,or with different IPTG concentrations and with different induction time,SDS-PAGE and Western blot were used to analyze expression and antigenicity of rVP2.The results showed that the molecular weight of the rVP2 was about 72 ku,the protein existed in the form of inclusion body under different conditions.The expression levels showed the highest with 0.2mmoL/L final concentration of IPTG,induced for 4 hat 37 ℃.rVP2 can not only react with His-tag monoclonal antibody but also can react with specific positive serum of CPV,rVP2 showed good reactogenicity.This study laid the foundation for the development of CPV antibody detection kits and genetic vaccine." @default.
- W2372994370 created "2016-06-24" @default.
- W2372994370 creator A5017465645 @default.
- W2372994370 date "2015-01-01" @default.
- W2372994370 modified "2023-09-23" @default.
- W2372994370 title "Prokaryotic Expression and Reactogenicity Identification of Canine Parvovirus NY Strain VP2 Protein" @default.
- W2372994370 hasPublicationYear "2015" @default.
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