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- W2372994871 abstract "Objective To prepare nerve growth factor (NGF) antiserum and investigate its primary application. Methods NGF was used to immunize the New Zealand rabbit and antibody against NGF was prepared. The high titre of antiserum was gained and radioimmunoassay (RIA) for NGF detecting radioimmunoassay (RIA) was established. Results The NGF antiserum possesses high titre (1∶20000) and high affinity (K=1.66×10 -10 mol//L ) . The specificity of NGF antiserum was high and it did not react with GDNF, CNTF, EGF, β-EP, CCK, GHRH, GH, BNP or Bambsin. The plasma level of NGF in depression people was (360.05±51.89) pg/ml while that of control was (313.53±34.02) pg/ml . The NGF level in mouse submaxillary glands was (202.09±32.61) ng/mg. Conclusion The NGF anti-serum we gained possesses high titre, high affinity and high specificity. RIA of NGF we established is delicate, simple and rapid. It is a reliable methed for studying the role of NGF in various physiological and pathophysiological conditions." @default.
- W2372994871 created "2016-06-24" @default.
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- W2372994871 date "2004-01-01" @default.
- W2372994871 modified "2023-09-26" @default.
- W2372994871 title "Preparation and application of nerve growth factor antiserum" @default.
- W2372994871 hasPublicationYear "2004" @default.
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