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- W2373062938 abstract "Objective: To construct a method to research the gene FM-STS' function that the gene come by RACE amplified from the stilbene synthase gene's consensus sequence in Polygonum multiflorum Thunb.Method: The over-expression vector pBIN-35S-STS-GFP was constructed from the blank plasmids pBIN-35S-GFP and the gene FM-STS sequence and also the interference expression vector pBIN-35S-forward-reverse-GFP was constructed from the blank plasmids and the forward/ reverse direction sequence on account of double stranded RNA.Sent the over-expression plasmid/the interference plasmid and the blank plasmid into wild-type Agrobacterium rhizogenes ATCC15834,then induced hairy roots and cultured them.In the end the hairy roots were analyzed by HPLC and Real-time PCR.Result: The PCR results showed that the gene rolB and gfp are both expressed in hairy roots,and the content of stilbene glucoside in blank group is 2.18mg/g,in the over-expression group is 4.67mg/g and in the interference group is 0.65mg/g,At the mRNA level to detect gene Fm-STS expression level,in the over-expression group is the highest,it's 3.17 times in the blank group and 101.44 times in the interference group.Conclusion: The method binding both technique that double stranded RNA-mediated gene silencing and over-expression is made good use of researching the gene' s function,and the gene FM-STS is the key enzyme gene in synthesis stilbene glucoside." @default.
- W2373062938 created "2016-06-24" @default.
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- W2373062938 date "2012-08-25" @default.
- W2373062938 modified "2023-09-24" @default.
- W2373062938 title "Double-stranded RNA-mediated Gene Silencing and Over-expression of FmSTS in Polygonum multiflorum Thunb Hairy Roots" @default.
- W2373062938 hasPublicationYear "2012" @default.
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